Coding

Part:BBa_K3039002:Design

Designed by: Ceilidh Welsh   Group: iGEM19_Exeter   (2019-08-29)


PETase T88A_S93M_S121E_W159F_D186H_R280A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 255
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was submitted to Twist Bioscience who codon optimised the sequence for E. coli, ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for potential TypeIIS assembly.


Source

Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by TWIST biosciences.

References